Hierarchical Surface Pattern on Ni‐Free Ti‐Based Bulk Metallic Glass to Control Cell Interactions

Fei‐Fan Cai, Andreu Blanquer, Miguel B. Costa, Lukas Schweiger, Baran Sarac, A. Lindsay Greer, Jan Schroers, Christian Teichert, Carme Nogués, Florian Spieckermann, Jürgen Eckert

Publikation: Beitrag in FachzeitschriftArtikelForschungBegutachtung


Ni-free Ti-based bulk metallic glasses (BMGs) are exciting materials for biomedical applications because of their outstanding biocompatibility and advantageous mechanical properties. The glassy nature of BMGs allows them to be shaped and patterned via thermoplastic forming (TPF). This work demonstrates the versatility of the TPF technique to create micro- and nano-patterns and hierarchical structures on Ti40Zr10Cu34Pd14Sn2 BMG. Particularly, a hierarchical structure fabricated by a two-step TPF process integrates 400 nm hexagonal close-packed protrusions on 2.5 µm square protuberances while preserving the advantageous mechanical properties from the as-cast material state. The correlations between thermal history, structure, and mechanical properties are explored. Regarding biocompatibility, Ti40Zr10Cu34Pd14Sn2 BMGs with four surface topographies (flat, micro-patterned, nano-patterned, and hierarchical-structured surfaces) are investigated using Saos-2 cell lines. Alamar Blue assay and live/dead analysis show that all tested surfaces have good cell proliferation and viability. Patterned surfaces are observed to promote the formation of longer filopodia on the edge of the cytoskeleton, leading to star-shaped and dendritic cell morphologies compared with the flat surface. In addition to potential implant applications, TPF-patterned Ti-BMGs enable a high level of order and design flexibility on the surface topography, expanding the available toolbox for studying cell behavior on rigid and ordered surfaces.
PublikationsstatusElektronische Veröffentlichung vor Drucklegung. - 18 Dez. 2023

Bibliographische Notiz

Funding Information:
This work was supported by the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska‐Curie grant agreement No. 861046 (BIOREMIA‐ETN). B.S. J.E. and F.S. acknowledge support from the Austrian Science Fund (FWF), Grant/Award Number: I3937‐N36, and A.B. and C.N. acknowledge support from the Spanish Government grant PID2020‐116844RB‐C21 and the Generalitat de Catalunya (2021‐SGR‐00122). J.S. was supported by the National Science Foundation through the Advanced Manufacturing Program (CMMI 2311311). The authors thank Dr. Sungwoo Sohn, Naijia Liu, and Arindam Raj for their insightful scientific discussions regarding thermomechanical molding techniques. Further thanks to Robin Neubauer and Michael Pegritz for their practical opinions on the TPF design, and Dr. Michael Burtscher for his recommendations on cell analysis software. A.B. and C.N. would like to thank the staff from the Servei de Microscòpia of Universitat Autònoma de Barcelona.

Publisher Copyright:
© 2023 The Authors. Small published by Wiley-VCH GmbH.

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