Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

Stefan Neubauer, Ariana Rugova, Dinh Binh Chu, Hedda Drexler, Anja Ganner, Michael Sauer, Diethard Mattanovich, Stephan Hann, Gunda Koellensperger

Publikation: Beitrag in FachzeitschriftArtikelForschungBegutachtung

27 Zitate (Scopus)


In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.
Seiten (von - bis)799-808
FachzeitschriftAnalytical and bioanalytical chemistry
PublikationsstatusVeröffentlicht - Aug. 2012

Bibliographische Notiz

Funding Information:
Acknowledgements This work was supported by the Biomin B.R.A.I.N program and by the Austrian Research Promotion Agency FFG (FHplus projekt METORGANIC).

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