Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

Stefan Neubauer; Ariana Rugova; Dinh Binh Chu; Hedda Drexler; Anja Ganner; Michael Sauer; Diethard Mattanovich; Stephan Hann; Gunda Koellensperger

doi:10.1007/s00216-012-6170-9

Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

Stefan Neubauer
, Ariana Rugova
, Dinh Binh Chu
, Hedda Drexler
, Anja Ganner
, Michael Sauer
, Diethard Mattanovich
, Stephan Hann
, Gunda Koellensperger

Lehrstuhl für Allgemeine und Analytische Chemie (120)

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Begutachtung

27 Zitate (Scopus)

Abstract

In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

Originalsprache	Englisch
Seiten (von - bis)	799-808
Seitenumfang	10
Fachzeitschrift	Analytical and bioanalytical chemistry
Jahrgang	404.2012
Ausgabenummer	3
DOIs	https://doi.org/10.1007/s00216-012-6170-9
Publikationsstatus	Veröffentlicht - Aug. 2012

Bibliographische Notiz

Funding Information:
Acknowledgements This work was supported by the Biomin B.R.A.I.N program and by the Austrian Research Promotion Agency FFG (FHplus projekt METORGANIC).

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title = "Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements",

abstract = "In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 \% aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.",

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note = "Funding Information: Acknowledgements This work was supported by the Biomin B.R.A.I.N program and by the Austrian Research Promotion Agency FFG (FHplus projekt METORGANIC).",

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AU - Ganner, Anja

AU - Sauer, Michael

AU - Mattanovich, Diethard

AU - Hann, Stephan

AU - Koellensperger, Gunda

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N2 - In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

AB - In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

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KW - LC-ICP-MS

KW - LC-MS-MS

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