Systems-level organization of yeast methylotrophic lifestyle

Hannes Rußmayer; Markus Buchetics; Clemens Gruber; Minoska Valli; Karlheinz Grillitsch; Gerda Modarres; Raffaele Guerrasio; Kristaps Klavins; Stefan Neubauer; Hedda Drexler; Matthias G. Steiger; Christina Troyer; Ali Al Chalabi; Guido Krebiehl; Denise Sonntag; Günther Zellnig; Günther Daum; Alexandra B. Graf; Friedrich Altmann; Gunda Koellensperger; Stephan Hann; Michael Sauer; Diethard Mattanovich; Brigitte Gasser

doi:10.1186/s12915-015-0186-5

Systems-level organization of yeast methylotrophic lifestyle

Hannes Rußmayer
, Markus Buchetics
, Clemens Gruber
, Minoska Valli
, Karlheinz Grillitsch
, Gerda Modarres
, Raffaele Guerrasio
, Kristaps Klavins
, Stefan Neubauer
, Hedda Drexler
, Matthias G. Steiger
, Christina Troyer
, Ali Al Chalabi
, Guido Krebiehl
, Denise Sonntag
, Günther Zellnig
, Günther Daum
, Alexandra B. Graf
, Friedrich Altmann
, Gunda Koellensperger

Stephan Hann, Michael Sauer, Diethard Mattanovich, Brigitte Gasser

Universität für Bodenkultur Wien : Standort Wien
Austrian Centre of Industrial Biotechnology GmbH
University of Applied Sciences FH Campus
Sandoz GmbH
Biocrates Life Sciences AG
Universität Tübingen
Technische Universität Graz
Universität Wien

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Begutachtung

97 Zitate (Scopus)

Abstract

Some yeasts have evolved a methylotrophic lifestyle enabling them to utilize the single carbon compound methanol as a carbon and energy source. Among them, Pichia pastoris (syn. Komagataella sp.) is frequently used for the production of heterologous proteins and also serves as a model organism for organelle research. Our current knowledge of methylotrophic lifestyle mainly derives from sophisticated biochemical studies which identified many key methanol utilization enzymes such as alcohol oxidase and dihydroxyacetone synthase and their localization to the peroxisomes. C1 assimilation is supposed to involve the pentose phosphate pathway, but details of these reactions are not known to date.

Originalsprache	Englisch
Aufsatznummer	80
Seitenumfang	25
Fachzeitschrift	BMC biology
Jahrgang	13.2015
Ausgabenummer	1
DOIs	https://doi.org/10.1186/s12915-015-0186-5
Publikationsstatus	Veröffentlicht - 23 Sept. 2015
Extern publiziert	Ja

Bibliographische Notiz

Funding Information:
This work has been supported by the Federal Ministry of Science, Research and Economy (BMWFW), the Federal Ministry of Traffic, Innovation and Technology (bmvit), the Styrian Business Promotion Agency SFG, the Standortagentur Tirol, the Government of Lower Austria and ZIT – Technology Agency of the City of Vienna through the COMET-Funding Program managed by the Austrian Research Promotion Agency FFG. Further support by Biomin Research Center, Boehringer-Ingelheim RCV, Lonza AG, Biocrates Life Sciences AG, VTU Technology GmbH, and Sandoz GmbH is acknowledged. EQ BOKU VIBT GmbH is acknowledged for providing mass spectrometry and bioreactor instrumentation. The authors are grateful to Amy A. Caudy (University of Toronto, Canada) for kindly providing a standard of seduheptulose bisphosphate and thank Jonas Hohlweg for excellent support in transcriptomics analyses.

Publisher Copyright:
© 2015 Rußmayer et al.

Schlagwörter

Analytische Chemie

Zugriff auf Dokument

10.1186/s12915-015-0186-5

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Verknüpfung zur Publikation in Scopus

Dieses zitieren

Rußmayer, H., Buchetics, M., Gruber, C., Valli, M., Grillitsch, K., Modarres, G., Guerrasio, R., Klavins, K., Neubauer, S., Drexler, H., Steiger, M. G., Troyer, C., Al Chalabi, A., Krebiehl, G., Sonntag, D., Zellnig, G., Daum, G., Graf, A. B., Altmann, F., ... Gasser, B. (2015). Systems-level organization of yeast methylotrophic lifestyle. BMC biology, 13.2015(1), Artikel 80. https://doi.org/10.1186/s12915-015-0186-5

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abstract = "Some yeasts have evolved a methylotrophic lifestyle enabling them to utilize the single carbon compound methanol as a carbon and energy source. Among them, Pichia pastoris (syn. Komagataella sp.) is frequently used for the production of heterologous proteins and also serves as a model organism for organelle research. Our current knowledge of methylotrophic lifestyle mainly derives from sophisticated biochemical studies which identified many key methanol utilization enzymes such as alcohol oxidase and dihydroxyacetone synthase and their localization to the peroxisomes. C1 assimilation is supposed to involve the pentose phosphate pathway, but details of these reactions are not known to date.",

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Rußmayer, H, Buchetics, M, Gruber, C, Valli, M, Grillitsch, K, Modarres, G, Guerrasio, R, Klavins, K, Neubauer, S, Drexler, H, Steiger, MG, Troyer, C, Al Chalabi, A, Krebiehl, G, Sonntag, D, Zellnig, G, Daum, G, Graf, AB, Altmann, F, Koellensperger, G, Hann, S, Sauer, M, Mattanovich, D & Gasser, B 2015, 'Systems-level organization of yeast methylotrophic lifestyle', BMC biology, Jg. 13.2015, Nr. 1, 80. https://doi.org/10.1186/s12915-015-0186-5

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AU - Buchetics, Markus

AU - Gruber, Clemens

AU - Valli, Minoska

AU - Grillitsch, Karlheinz

AU - Modarres, Gerda

AU - Guerrasio, Raffaele

AU - Klavins, Kristaps

AU - Neubauer, Stefan

AU - Drexler, Hedda

AU - Steiger, Matthias G.

AU - Troyer, Christina

AU - Al Chalabi, Ali

AU - Krebiehl, Guido

AU - Sonntag, Denise

AU - Zellnig, Günther

AU - Daum, Günther

AU - Graf, Alexandra B.

AU - Altmann, Friedrich

AU - Koellensperger, Gunda

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AU - Sauer, Michael

AU - Mattanovich, Diethard

AU - Gasser, Brigitte

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