The groundwater of the west Assiut and El-Minia districts was evaluated in this paper using geoelectrical, hydrogeochemical, and stable isotope (oxygen-18 and deuterium) studies. In the studied localities, 42 vertical electrical soundings (VES) were taken to evaluate groundwater potential, and the analysis, as well as collection, of 74 samples of Eocene groundwater was carried out. In accordance with the vertical electrical soundings’ interpretation, there are four geoelectrical formations and two major water-bearing units that act as aquifers (Pleistocene and Eocene). To determine irrigation suitability, the sodium absorption ratio (SAR), electrical conductivity (EC), residual sodium carbonate (RSC), sodium percentage (Na %), magnesium hazard (MH), Kelley’s ratio (KR), and permeability index (PI) were evaluated as irrigation quality parameters. The EC, Na %, and the diagram of the US salinity laboratory indicated that most of the collected samples of groundwater were suitable for irrigation, whereas the RSC and PI pointed out that all of the collected water samples were safe for irrigation. The oxygen and hydrogen isotope values in groundwater samples showed that the Eocene aquifer was recharged by both surface water and the Nubian aquifer.