Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

Stefan Neubauer; Ariana Rugova; Dinh Binh Chu; Hedda Drexler; Anja Ganner; Michael Sauer; Diethard Mattanovich; Stephan Hann; Gunda Koellensperger

doi:10.1007/s00216-012-6170-9

Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

Stefan Neubauer, Ariana Rugova, Dinh Binh Chu, Hedda Drexler, Anja Ganner, Michael Sauer, Diethard Mattanovich, Stephan Hann, Gunda Koellensperger

Chair of General and Analytical Chemistry (120)

Research output: Contribution to journal › Article › Research › peer-review

27 Citations (Scopus)

Abstract

In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

Original language	English
Pages (from-to)	799-808
Number of pages	10
Journal	Analytical and bioanalytical chemistry
Volume	404.2012
Issue number	3
DOIs	https://doi.org/10.1007/s00216-012-6170-9
Publication status	Published - Aug 2012

Keywords

Feed supplemen
LC-ICP-MS
LC-MS-MS
Nucleobase
Nucleoside
Nucleotide

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10.1007/s00216-012-6170-9

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title = "Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements",

abstract = "In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.",

keywords = "Feed supplemen, LC-ICP-MS, LC-MS-MS, Nucleobase, Nucleoside, Nucleotide",

author = "Stefan Neubauer and Ariana Rugova and Chu, {Dinh Binh} and Hedda Drexler and Anja Ganner and Michael Sauer and Diethard Mattanovich and Stephan Hann and Gunda Koellensperger",

note = "Funding Information: Acknowledgements This work was supported by the Biomin B.R.A.I.N program and by the Austrian Research Promotion Agency FFG (FHplus projekt METORGANIC).",

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AU - Neubauer, Stefan

AU - Rugova, Ariana

AU - Chu, Dinh Binh

AU - Drexler, Hedda

AU - Ganner, Anja

AU - Sauer, Michael

AU - Mattanovich, Diethard

AU - Hann, Stephan

AU - Koellensperger, Gunda

N1 - Funding Information: Acknowledgements This work was supported by the Biomin B.R.A.I.N program and by the Austrian Research Promotion Agency FFG (FHplus projekt METORGANIC).

PY - 2012/8

Y1 - 2012/8

N2 - In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

AB - In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

KW - Feed supplemen

KW - LC-ICP-MS

KW - LC-MS-MS

KW - Nucleobase

KW - Nucleoside

KW - Nucleotide

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DO - 10.1007/s00216-012-6170-9

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SN - 1618-2642

VL - 404.2012

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JO - Analytical and bioanalytical chemistry

JF - Analytical and bioanalytical chemistry

IS - 3

ER -

Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

Abstract

Keywords

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