Systems-level organization of yeast methylotrophic lifestyle

Hannes Rußmayer, Markus Buchetics, Clemens Gruber, Minoska Valli, Karlheinz Grillitsch, Gerda Modarres, Raffaele Guerrasio, Kristaps Klavins, Stefan Neubauer, Hedda Drexler, Matthias G. Steiger, Christina Troyer, Ali Al Chalabi, Guido Krebiehl, Denise Sonntag, Günther Zellnig, Günther Daum, Alexandra B. Graf, Friedrich Altmann, Gunda KoellenspergerStephan Hann, Michael Sauer, Diethard Mattanovich, Brigitte Gasser

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97 Citations (Scopus)


Some yeasts have evolved a methylotrophic lifestyle enabling them to utilize the single carbon compound methanol as a carbon and energy source. Among them, Pichia pastoris (syn. Komagataella sp.) is frequently used for the production of heterologous proteins and also serves as a model organism for organelle research. Our current knowledge of methylotrophic lifestyle mainly derives from sophisticated biochemical studies which identified many key methanol utilization enzymes such as alcohol oxidase and dihydroxyacetone synthase and their localization to the peroxisomes. C1 assimilation is supposed to involve the pentose phosphate pathway, but details of these reactions are not known to date.
Original languageEnglish
Article number80
Number of pages25
JournalBMC biology
Issue number1
Publication statusPublished - 23 Sept 2015
Externally publishedYes

Bibliographical note

Publisher Copyright: © 2015 Rußmayer et al.


  • Metabolome
  • Methanol
  • Peroxisome
  • Pichia pastoris
  • Proteome
  • Transcriptome
  • Xylulose-monophosphate cycle

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